5 × rt buffer and m-mlv rt 50.000 u (Promega)
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5 × rt buffer and m-mlv rt 50.000 u
5 × Rt Buffer And M Mlv Rt 50.000 U, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
5 × Rt Buffer And M Mlv Rt 50.000 U, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+rt+buffer/5+%C3%97+rt+buffer+and+m+mlv+rt+50+000+u/pm40373708-115-17-21
Average 90 stars, based on 1 article reviews
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Reverse Transcription:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Incubation:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Polymerase Chain Reaction:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of cDNA Synthesis:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Real-time Polymerase Chain Reaction:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Virus:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of FACS:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Lysis:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Fluorescence:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of Sterility:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: A double-hit in vivo model of GBA viral microRNA-mediated downregulation and human alpha-synuclein overexpression demonstrates nigrostriatal degeneration. Article Snippet: For the cDNA synthesis, we used 5 μL of 5× Article Title: The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Article Snippet: Reprod Dom Anim 2016; 1–8 wileyonlinelibrary.com/journal/rda | 1© 2016 Blackwell Verlag GmbH Received: 4 May 2016 | Accepted: 29 August 2016 DOI: 10.1111/rda.12813 Contents In the horse, breeding induces a transient endometrial inflammation.. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating- induced endometritis PMIE Select seminal plasma proteins cysteine- rich secretory protein- 3 (CRISP- 3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro.. The objective of this study was to determine whether the addition of CRISP- 3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Article Title: Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay Article Snippet: Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 μl volume containing 7.5 μl eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 μl 5× Article Title: Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3 Article Snippet: Subsequently, 5 μl of 5× Article Title: Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis Article Snippet: Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 μl reverse transcription master mix was then added to each Article Title: Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts Article Snippet: Subsequently, the cDNA was synthesized using 4 μL of 5× RT buffer, 5 μL of 2 mM deoxynucleotide (dNTP), 0.5 μL of |